A β-D-glucose ligand equipped with a primary amine through a hydrophilic PEG3 spacer. The amine couples with NHS-activated esters to form stable amide bonds, providing a route to conjugate the ligand onto NHS-activated molecules and surfaces. This compound provides a β-D-glucose building block for glycoconjugate synthesis, glycomimetic research, and the construction of probes for glucose-recognizing proteins and lectins.