A tri-LacNAc biantennary N-glycan on an Asn core equipped with a biotin tag through a hydrophilic PEG8 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound presents a tri-LacNAc biantennary N-glycan on an Asn core, displaying extended poly-LacNAc arms that are high-affinity galectin ligands. The biotin tag enables immobilization on streptavidin/neutravidin-coated platforms for binding studies and glycan arrays.