A β-N-acetyllactosamine (LacNAc) ligand equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound provides an N-acetyllactosamine (LacNAc; Galβ1-4GlcNAc) building block, a core disaccharide of N- and O-glycans and a ligand for galectins. It is a substrate for sialyltransferases and fucosyltransferases, enabling enzymatic extension to sialylated and fucosylated structures such as sialyl-LacNAc and Lewis antigens.