A Man5GlcNAc2 high-mannose N-glycan on an Asn core equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound presents a Man5GlcNAc2 high-mannose N-glycan on an Asn core, a key biosynthetic intermediate and a ligand for mannose-recognizing receptors including the mannose receptor and DC-SIGN. The biotin tag enables immobilization on streptavidin/neutravidin-coated platforms for binding studies and glycan arrays.