An α-D-mannose ligand equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound provides an α-D-mannose building block for glycoconjugate synthesis and the construction of probes for mannose-recognizing receptors and lectins, including the mannose receptor (CD206) and DC-SIGN, with relevance to immune targeting and pathogen-recognition research.