A β-D-glucuronic acid (GlcA) 4-methylumbelliferyl (3MU) glycoside bearing a biotin tag. The biotin moiety binds with high affinity to streptavidin and neutravidin for immobilization, while the methylumbelliferyl aglycone provides a fluorogenic readout upon enzymatic cleavage. This compound is a 4-methylumbelliferyl (3MU) β-D-glucuronide bearing a biotin tag, serving as a fluorogenic substrate for β-glucuronidase and a probe for glucuronidase activity studies; the biotin enables immobilization on streptavidin/neutravidin-coated platforms.