A β-D-glucose ligand equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound provides a β-D-glucose building block for glycoconjugate synthesis, glycomimetic research, and the construction of probes for glucose-recognizing proteins and lectins.