A β-D-N-acetylglucosamine (GlcNAcβ) ligand equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound provides a β-N-acetylglucosamine (GlcNAcβ) building block for glycoconjugate synthesis and the construction of probes for GlcNAc-recognizing proteins, including lectins and antibodies. It is also relevant to O-GlcNAc and N-glycan biosynthesis research and serves as a substrate for galactosyltransferases and other glycosyltransferases.