A tri-antennary 6'-sialyl-LacNAc (6SLN3) N-glycan on an Asn core equipped with a biotin tag through a PEG8 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound presents a tri-antennary 6'-sialyl-LacNAc (6SLN3) N-glycan on an Asn core with a PEG8-biotin tag, displaying multivalent α2-6 sialosides that are ligands for Siglecs and human-type influenza hemagglutinin. The biotin tag enables immobilization on streptavidin/neutravidin-coated platforms.