An A2 biantennary disialo (α2-6) N-glycan equipped with a biotin tag through a 6-aminohexanoic acid spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound presents an A2 biantennary N-glycan capped with α2-6-linked sialic acid (disialo) on an Asn core, mimicking native complex-type N-glycans. α2-6 sialosides are ligands for Siglecs and human-type influenza hemagglutinin. The biotin tag enables immobilization on streptavidin/neutravidin-coated platforms for binding studies and glycan arrays.