A chitobiose (GlcNAcβ1-4GlcNAc) ligand equipped with a dibenzocyclooctyne (DBCO) handle through a propyl-PEG4 spacer. The DBCO group reacts with azide-functionalized partners via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC), forming stable 1,2,3-triazole linkages without a copper catalyst. This compound provides a chitobiose (GlcNAcβ1-4GlcNAc) building block, the core disaccharide of N-linked glycans, useful for studying N-glycan biosynthesis, chitinases, and GlcNAc-binding lectins.