A β-D-glucose ligand equipped with a thioacetate-protected thiol linker through a short ethyl spacer. The thioacetate serves as a masked thiol that can be deprotected under mild basic or hydrolytic conditions to reveal a free thiol, which can then be reacted with maleimides to form thioether conjugates or with thiol-modified substrates to form disulfide bonds. This compound provides a β-D-glucose building block for glycoconjugate synthesis, glycomimetic research, and the construction of probes for glucose-recognizing proteins and lectins.