A β-D-glucose ligand equipped with a thiol-functionalized linker through a short ethyl spacer. The thiol group can be reacted with other thiol-modified substrates to form disulfide bonds, or reacted with maleimides to form stable thioether conjugates. This compound provides a β-D-glucose building block for glycoconjugate synthesis, glycomimetic research, and the construction of probes for glucose-recognizing proteins and lectins.