A α2-6-sialylated tri-LacNAc glycan equipped with a biotin tag through a hydrophilic PEG3 spacer. The biotin moiety binds with high affinity to streptavidin and neutravidin, enabling straightforward immobilization on chips, in wells, or on other streptavidin/neutravidin-coated platforms. This compound presents α2-6-sialylated tri-LacNAc, a multivalent α2-6 sialoside that is a ligand for Siglecs and human-type influenza hemagglutinin. The biotin tag enables immobilization on streptavidin/neutravidin-coated platforms for binding studies.