A cleavable Biotin Probe for Labeling of Biomolecules via Azide–Alkyne Cycloaddition (Click Chemistry). The Biotin moiety is linked via a tetraethylene glycol (PEG4) spacer and cleavable dialkoxydiphenylsilane (DADPS) linker to an imidazolium-azide for click chemistry. The stable imidazolium cation increases the ionisation efficiency of glycopeptides by mass spectroscopy and enabling easier profiling over use of conventional picolyl-azide based tags.
