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Analytical Services

Sussex Research offers analytical and bioanalytical services on a fee-for-service basis – from glycoanalysis in support of structure characterization to small molecule characterization and bioanalytical analysis in support of drug discovery and research programs.

Bioanalytical Services

Sussex Research is a trusted analytical partner in pharmaceutical, agricultural and other research areas. We meet the challenge of providing rapid turn-around of quality bioanalytical results to facilitate the advancement of research programs. Sussex Research has extensive experience in the bioanalysis of small molecules and natural compounds and draws on this experience to develop custom assays and provide routine sample analysis.

Client communication is key in our ability to provide responsive, flexible and timely bioanalytical services. We provide continuous and open communication during method development and/or sample analysis and provide full reports upon completion of each bioanalytical study.

We employ LC-MS/MS, LC-MS and HPLC techniques in our analytical methodology.

Sussex Research provides bioanalytical support to facilitate the decision making process in pharmaceutical discovery. We leverage our expertise in the discovery arena to reduce method development times and provide rapid turn-around of samples results for lead optimization programs.

Pharmaceutical Discovery Support

Services Offered

Method Development: LC-MS/MS quantitative method development is provided for single compound, cassette doses or compound series to support in-vitro and in-vivo studies. Generic extraction and chromatographic techniques coupled to LC-MS/MS detection result in rapid method development to discovery stage appropriate specifications.

Sample Analysis: LC-MS/MS methods are employed in the analysis of in-vitro and in-vivo samples. Sample matrix and calibration ranges are flexible and are tailored to meet individual study requirements. Results are reported as test compound concentration per sample (ng/mL) in Excel format.

Plasma Stability: Test article stability (10 µg/mL or other concentrations) is determined in plasma (various species and anti-coagulants). Incubation of test article(s) in matrix at room temp and/or 37˚C with sampling at T0, 15m, 1h, and 4h (parameters may be tailored to study). LC-MS/MS analysis is used to determine sample concentrations with results reported as % of Time 0 samples. Stability evaluation in solvents, solutions and dosing solutions is also available.

Plasma Protein Binding: Test article binding to plasma proteins is determined (various species and anti-coagulants) via incubation of test article(s) in matrix at 37˚C followed by ultra-centrifugation. Non-specific binding to filters is evaluated. LC-MS/MS analysis is used to determine the total and free concentrations of test article in pre- and post-filtered plasma. % protein binding is calculated and results are reported as % bound.

Carbohydrate Analysis

Researchers often require knowledge of the types and concentrations of small carbohydrates in biological systems to provide insight into the relationships and function of this class of compounds in those systems.

Sussex Research provides quantitative analysis of selected small reducing and non-reducing carbohydrates in biological samples via LC-MS/MS. We have established in-house methods for aqueous-based sample matricies and are able to adapt the methods to other matricies as required. The achievable lower limits of quantitation (LLOQ) are in the low ng/mL (ppb) range with LLOQ’s and calibration ranges tailored to specific studies. If desired, the methods may also be modified to include other carbohydrates for quantitative or qualitative purposes.

Amino Acid Analysis

Researchers often require knowledge of the types and concentrations of free amino acids in biological systems to provide insight into the relationships and function of this class of compounds in those systems.

Sussex Research provides quantitative analysis of the 20 naturally occuring proteinogenic amino acids in biological samples via LC-MS/MS. We have established in-house methods for aqueous-based sample matricies and are able to adapt the methods to other matricies as required. The achievable lower limits of quantitation (LLOQ) are in the low ng/mL (ppb) range with LLOQ’s and calibration ranges tailored to specific studies. If desired, the methods may also be modified to include non-proteinogenic amino acids for quantitative or qualitative purposes.

Fatty Acid Analysis

Researchers often require knowledge of the types and concentrations of free fatty acids in biological systems to provide insight into the relationships and function of this class of compounds in those systems.

Sussex Research provides quantitative analysis of several medium to long chain fatty acids in biological samples via LC-MS/MS. We have established in-house methods for aqueous-based sample matricies and are able to adapt the methods to other matricies as required. The achievable lower limits of quantitation (LLOQ) are in the low-mid ng/mL (ppb) range with LLOQ’s and calibration ranges tailored to specific studies. If desired, the methods may also be modified to include other fatty acids for quantitative or qualitative purposes.

Analytical Services

Sussex Research offers compound purification and non-GMP certificate of analysis (CofA) for new chemical entities (NCE’s) as well as small molecules and peptides.

Services Offered

Prep-HPLC purification of small molecules and peptides

CofA (non-GMP): May include NMR, Mass, HPLC-UV purity, FT-IR and/or Optical rotation

Structural Analysis of Carbohydrates

Sussex Research offers comprehensive services for structural analysis of carbohydrates.

Our Glycoanalysis Laboratory draws on its extensive experience in high-resolution and solid-state Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS) and leading expertise in the purification and chemical characterization of glycans.